Amino Acids on the Chromatogram

At present there is no known specific reagent for amino acids alone. Some non-specific reagents for nitrogen-contammg compounds and 

The usual vacuum-drying ovens with heating are quite expensive. 

Esseb [210] describes a quantitative determination of the ninhydrin-positive compounds separated on cellulose powder MN 300. 

The dried plates are briefly immersed in a 0.6% solution of ninhydrin in acetone and then maintained for 105 min at 70° C. The zones are immediately scraped off, mixed with 0.4 ml of a 0.5% solution of cadmium acetate in methanol and 

Two-dimensional chromatogram of the amino acid mixture as shown in Fig. 206 

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