Amines derivatizing

Wash the amine-derivatized particles at least 3 times with water using centrifugation to remove excess reactants. 

Primary amines Derivatization with divinyl sulfone, [Ru(bpy)3]2+ ECL 1-30 pmol 74... 

The surfaces included PLL, polystyrene, epoxy-terminated polyethylene glycol (PEG) or dendrimer slides, various amine-derivatized surfaces, and nitrocellulose-coafed slides. All proteins were printed in PBS, rinsed in TBS, and then blocked in 3% nonfat dry milk powder dissolved in TBS-0.1% Tween-20. A final rinse in TBS was performed prior to incubation. While no attempt was made to optimize print buffer or blocking conditions for each of the selected surfaces, if was apparenf fhaf wifh fhe exception of activated polystyrene, most chemistries performed af abouf fhe same levels, i.e., within two- to threefold af saturation. 

Another approach would be to derivatize cyclamate prior to analysis. Cyclamate can be determined by HPLC and UV detection at 314 nm after conversion to A/,/V-dichlorocyclohexyl-amine. Derivatization can be carried out directly in the sample or after extraction and cleanup. /V,/V-Dichlorocyclohexylamine is separated on a reverse-phase column (Nucleosil Cl8 or Fine-pak SIL Cl8 T-5) with a mobile phase of methanol water, 8 2 v/v (43,46). Cyclamate can also be determined at 585 nm after postcolumn derivatization with methyl violet 2B as described by Lawrence and Charbonneau (16). 

The Development and Application of Tetrafluorophenol-Activated Resins for Rapid Amine Derivatization... 

Amine Derivatization with TFP-Activated Esters Reagents... 

Hydrochloride may be converted back to the free amine derivatizing agent prior to use. This may be done by dissolving the salt in water, then adding 1 N NaOH soln. and extracting the free amine into toluene. The toluene phase is dried with Na O, and then diluted to 250 mL. The concentration of this amine solution is 0.002 M (Soln. A). This may be tenfold diluted to give 0.0002 M amine solution (Soln. B). 

Simple tertiary amines are difficult to deprotonate selectively [195, 196, 200], To increase the acidity of the a-C,H-groups the amine can be quaternized [207], treated with a Lewis acid [208-211], oxidized to an amine N-oxide[161], or, for secondary amines, derivatized with a functional group capable of forming a chelate with the metal (Scheme 5.22). 

Because carbon dioxide is nonpolar, the separation of polar compounds by supercritical carbon dioxide is difficult. Thus, polar modifiers are often used for the separation of phenols and amines. Derivatization has also been employed to obtain nonpolar analytes in some applications. The UV detector has mainly been used for the detection of polar compounds. Oxidative and reductive amperometric detection was also utilized with a detection limit of 250 pg for oxidative detection of 2,6-dimethylphenol. The detection of amines has generally been achieved by FID. Other detectors used for the detection of polar analytes include Fourier transform infrared (FTIR), photodiode array, and flame photometry. 

Fig. 4. Immobilization methods. (A) Attachment of biomolecules onto thiol-derivatized surfaces (see Subheading 3.2.1 for experimental details). (B) Attachment of biomolecules onto amine-derivatized surfaces (Subheading 3.2.2) using amine-amine linking (upper pathway, Subheading Amine-Amine (Homobifunctional Crosslinkers) ) and amine-thiol linking (lower pathway, Subheading Amine-Thiol Crosslinking (Heterobifunctional Crosslinkers) ). (C) Noncovalent attachment of gangliosides/globosides onto hydrophobic surfaces (Subheading 3.2.3),...

During this process, the hydroxyl groups on the surface of the slide react with the ethoxy groups of aminopropyl triethoxy silane resulting in an amine-derivatized slide (24). 

Tod, M., Legendre, J. Y., Chalom, J., Kouwath, H., Poulou, M., Farinotti, R., and Mahuzier, G., Ihimary and secondary amine derivatization with luminarins 1 and 2 separation hy liquid chromatography with peroxyoxalate chemiluminescence detection, J. Chromatogr., 594, 386-391,... 

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