Amine-Reactive Cascade Blue Acetyl Azide

Amine-Reactive Cascade Blue Acetyl Azide 

One Cascade Blue derivative is available for creating linkages with amine-containing molecules. The acetyl azide functionality of this reagent reacts with primary amines at ambient temperatures or below to create amide bond derivatives (Lanier and Recktenwald, 1991 Oparka et al., 

This fluorophore has excitation maxima at 375 and 400 nm and an emission maximum at 410 nm. The small Stoke s shift may create some difficulty in discrete excitation without contaminating the emission measurement with scattered or overlapping light. The extinction coefficient of the molecule in water is about 27,000M 1cm 1. Cascade Blue and Lucifer Yellow derivatives can be simultaneously excited by light of less than 400 nm, resulting in two-color detection at 410 and 530 nm. 

Cascade Blue acetyl azide is soluble in aqueous solution, but the reactive azide group will hydrolyze and should be used immediately in a conjugation reaction. A concentrated stock solution may be prepared in water, dissolved quickly, and an aliquot quickly added to a buffered reaction medium. For aqueous reactions, a pH range of 7-9 is optimal. Avoid amine-containing buffers. 

Caitoxylate-Reactive Cascade Blue Cadaverine and Cascade Blue Eth enediamine 

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Amine-Reactive Cascade Blue Acetyl Azide... 

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