Amidase from Pseudomonas fluorescens

In addition, at Ube an analogous process for the enantioselective synthesis of a-methyl phenylalanine has been developed based on an amidase from Pseudomonas fluorescens [IFO 3081] [14]. 

The processes are based on whole bacterial cells. In the case of the pipecolic acid, an important building block for pharmaceutical chemistry, an S-selective amidase in Pseudomonas fluorescens cells, catalyses the reaction with high selectivity and the acid is obtained with an ee >99% (Scheme 6.27A). For the preparation of piperazine-2-carboxylic acid from the racemic amide a R- and a S-selective amidase are available. Utilising Klebsiella terrigena cells the S-enantiomer is prepared with 42% isolated yield and ee > 99%, while Burkholderia sp. cells catalyse the formation of the -enantiomer (ee=99%, Scheme 6.27 B). 

Synthesis of (S)-mandelic acid using S-selective hydroxynitriie iyase (HNL) from Manihot escu-lenta, nonseiective nitriiase from Pseudomonas fluorescens EBC 191, and amidase from Rhodococcus erythropolis [55]. 

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