Alpha plot method

To evaluate the concentration of water-soluble protein, the Bradford method was used. The calibrating plot for evaluation of protein concentration was generated using alpha-chymotrypsin. 

Conductometric titration of alpha acids was in the not so distant past the most common procedure in the brewery laboratories. In this method, a suitable solution of an extracted hop or of the dissolved hop extract is titrated with a solution of lead(ll) acetate and the conductivity plot allows deduction of the results. Many contributions on the conductometry of alpha acids have appeared in the literature. The titration curve can be influenced substantially by changing the titration medium, as we found out for a number of mixed solvents (10), Current methods add either a base or dimethylsulfoxide to improve the determination of the titration endpoint. The Analysis Committee of the European Brewery Convention repeatedly organized collaborative trials to evaluate hop conductometric analysis. The efforts of a Working Group on Hop... 

---