Alkaline phosphatase chicken intestine

The type of inhibition of chicken intestine alkaline phosphatase by forphenicine was not competitive, but uncompetitive with the stibstrate. Its derivative, forphenicinol, which contains a hydroxymethyl grorp instead of the formyl group in the forphenicine molecule, did not inhibit alkaline phosphatase but, it did bind to cells. Forphenicinol enhanced delayed-type lOT>ersensiti-vity (13,14) and the phagocytic activity of macrophages. 

Anagnostopoulos found that the amino group reagents ketene, nitrous acid, formaldehyde, and phenyl isocyanate all inactivated bovine liver and kidney phosphatases 102). On the other hand, acetylation of chicken intestinal phosphatase with acetic anhydride gave an active product with optimum activity more alkaline than normal 103). The enzyme preparation was impure and acetylation only 70% complete... 

Perhaps the most characteristic feature of alkaline phosphatase is the way in which the pH optimum changes with increasing substrate concentration. A typical set of curves for calf intestinal phosphatase and phenyl phosphate is given in Fig. 1. Other examples of this type of behavior are found with -glycerophosphate and chicken intestinal... 

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