Assay algal

Laboratory algal assays for monitoring the toxicity of an allelochemical are very useful, due to the ease and rapidity with which the organism can be cultured and their responses measured. They also require less equipment, space and time of the researcher. 

Gueguen, C., Koukal, B., Dominik, J. and Pardos, M. (2003) Competition between alga (Pseudokirchneriella subcapitata), humic substances and EDTA for Cd and Zn control in the algal assay procedure (AAP) medium, Chemosphere 53 (8), 927-934. 

Vanderbroele, M.C., Heijerick, D.G., Vangheluwe, M.L. and Janssen, C.R. (2000) Comparison of the conventional algal assay and the Algaltoxkit F microbiotest for toxicity evaluation of sediment pore waters, in G. Persoone, C. Janssen and W. M. De Coen (eds.), New Microbiotests for Routine Toxicity Screening and Biomonitoring, Kluwer Academic / Plenum Publishers, New York, pp. 261-268. 

Because of the time and labor involved in algal assays, FRP (0.45 or 0.2 pm) has often been used as a surrogate for readily BAP, and TP (which includes the particulate, condensed, and organic phosphorus components) as a measure of potentially BAP. However, a number of studies have shown that neither FRP nor TP correlate well with algal assay-measured BAP. This may be because FRP tends to overestimate the true orthophosphate due to hydrolysis of labile organic and condensed species. Use of ultrafiltration through a low molecular mass filter prior to measurement of reactive phosphorus has been proposed as better estimator of BAP than other available chemical methods. 

The 6 rice herbicides (Bensulfiironmethyl, Imazosulfiiron, Simetryn, Dimethametryn, Pretilachlor and Cafenstrole) were used for the algal assay. 

By use of model substrates and inhibitor studies, an esterase that is reactive in unbuffered sea water as well as in the disrupted algal tissue from C. taxifolia was identified which mediates cleavage of the acetyl residues of caulerpenyne (54) [121]. After complete deacetylation, oxytoxin 2 (64) appears as an unstable end-product. Due to the lack of an appropriate assay procedure for labile metabo-... 

First of all, the allelopathic activity of a compound (or of a mixture of compounds) isolated as per the above methods should be tested in agar diffusion assays using different algal strains The selection of an assay alga depends primarily on its ability to grow satisfactory under the chosen... 

Kayser, H. 1976. Waste-water assay with continuous algal cultures the effect of mercuric acetate on the growth of some marine dinoflagellates. Mar. Biol. 36 61-72. 

Steinberg PD, Van Altena I (1992) Tolerance of marine invertebrate herbivores to brown algal phlorotannins in temperate Australasia. Ecol Monogr 62 189-222 Stem JL, Hagerman AE, Steinberg PD, Winter FC, Estes JA (1996) A new assay for quantifying brown algal phlorotannins and comparisons to previous methods. J Chem Ecol 22 1273-1293... 

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