Alcohol Gluconobacter oxydans

In case of primary alcohol substrates, biooxidation can also proceed to the carboxylic acid, enabling a facile separation of the chiral products by simple extraction. Whole-cells of Gluconobacter oxydans were utilized to produce S-2-phenylpro-panoic acid and R-2-phenylpropionic alcohol in excellent yields and optical purities (Scheme 9.4) [46]. 

Tkac et al. [16] Ethanol Off-line monitoring of S. cerevisiae batch fermentation Alcohol oxidase (AOx) in Gluconobacter oxydans cells/covered with a cellulose acetate (CA) membrane Glassy carbon electrode/ +300mV vs. SCE Ferricyanide... 

Figure 16.2-45. Preparation of isovaleraldehyde using an alcohol dehydrogenase (ADH) in whole cells of Gluconobacter oxydans.

Figure 16.2-47. Resolution of racemic (R,S)-2-phenylpropionic alcohol with whole cells of Gluconobacter oxydans yielding (S)-2-phenylpropanoic acid and (R)-2-phenylpropionic alcohol.

Besides the multigram-scale production of different aliphatic carboxylic acids by biocatalytic alcohol oxidation, especially the enantioselective oxidation of racemic 2-phenyl-l-propanol to (S)-2-phenylpropanoic acid with Gluconobacter oxydans (Fig. 16.2-47) is another good example of acid production from alcohols12091. 

Polypropylene/HF Microporous Oxidation of isoamyl alcohol with Gluconobacter oxydans cells [155]... 

Acetic acid fermentation Vinegar Grapes, potatoes, various fruits after alcoholic fermentation Acetobacter europeus Acetobacter aceti Acetobacter pasteurianus Acetobacter hansenii Gluconobacter oxydans... 

Gluconobacter oxydans is generally found growing in sugar-rich environments where alcohol is either absent or present in low concentrations. Typical sites of isolation include flower parts and deteriorating fruit. By comparison, Acetobacter spp. are generally isolated from fermented sub-... 

Hekmat D, Bauer R, Fricke J (2003) Optimization of the microbial synthesis of dihydroxyacetone from glycerol with Gluconobacter oxydans. Bioprocess Biosyst Eng 26 109-116 Huffer S, Clark ME, Ning JC, Blanch HW, Clark DS (2011) Role of alcohols in growth, lipid composition, and membrane fluidity of yeasts, bacteria, and archaetu Appl Environ Microbiol... 

Habe H, Fukuoka T, Morita T, Kitamoto D, Yakushi T, Matsushita K, Sakaki K (2010) Disruption of the membrane-bound alcohol dehydrogenase-encoding gene improved glycerol use and dihydroxyacetone productivity in Gluconobacter oxydans. Biosci Biotechnol Biochem 74 1391-1395... 

---