Aim and Relevance

The aim of this chapter is to design asymmetric ligands which enforce a 5,6-mixed coordination sphere similar to the active site in GpdQ. Two ligands will be discussed. One ligand, with a phenol, two pyridine donors, one tertiary and one 

Parts of this chapter have appeared in L.J. Daumann et al., Asymmetric Zinc(II) Complexes as Functional and Structural Models for Phosphoesterases Dalton Trans. 2013 42, 9574-9584— Reproduced by permission of The Royal Society of Chemistry. 

Daumann, Spectroscopic and Mechanistic Studies of Dinuclear Metallohydrolases 189 

7 Asymmetric Zn(II) Complexes as Stmctural and Functional Models for GpdQ 

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The validation exercises should demonstrate that the method delivers conclusions with a high degree of confidence in relation to the intended use of the finished product. Therefore, each study protocol should have clear aims and relevance. For example, if the method for measurement of surface contamination with antineoplastics is found to have a limit of detection of 5 ng/cm, while the intended patient dose is 1,000 mg, one may expect the data generated to meet the purpose of the study. One may question whether a very sensitive and costly HPLC/MS method provides relevant information or that a threshold has to be proposed as part of an AV study. 

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