Agrobacterium-mediated systems

Kapila, J. De Rycke, R. van Montagu, M. Angenon, G. Agrobacterium mediated transient gene expression system for intact leaves. Plant Sci. 1996, 122, 101-108. 

Johansen, L. K., J.C. Carrington. 2001. Silencing on the spot. Induction and suppression of RNA silencing in the Agrobacterium-mediated transient expression system. Plant Physiol 126, 930-938. 

About 20 years ago, it became possible to transfer foreign genes into the plant genome [60]. Since then, many attempts have been made to introduce new genes into plants to improve their quality. In this context, an Agrobacterium-mediated gene transfer system is widely used and successful results have been obtained in particular with regard to numerous solanaceous species. 

Michielse CB, Salim K, Ragas O, Ram AFJ, Kudla B, Jarry B, Punt PJ, van den Hondel CAMJJ. (2004). Development of a system for integrative and stable transformation of the Zygomycete Rhizopus oryzae by Agrobacterium-mediated DNA transfer. Mol Genet Genomics, 271,499-510. 

It is important to note that in some jurisdictions, the use of floral dip is restricted due to claims for using Agrobacterium-mediated transformation of any organ system in the intact plant as well as use in explant systems (EP 1334199, designated in Germany, Finland, France, Great Britain, and The Netherlands). This is an important consideration for commercialization of genetically modified CameUna. 

Marillonnet, S., Thoeringer, C., Kandzia, R., Klimyuk, V, and Gleba, Y. (2005). Systemic Agrobacterium tumefaciens-mediated transfection of viral repU-cons for efficient transient expression in plants. Nat. Biotechnol. 10 1038-. 

It was previously thought that Agrobacterium infection was limited almost exclusively to dicotyledon and gymnosperm species (92). Recent success in Agrdbacterium-mediated transformation of monocotyledon species such as Oryza sativa (93), Zea Mays (94), and Triticum aestivum (95) suggests that this may not be the case, although host range restrictions remain a limitation of this tians-foimation system. 

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