Aggregating primary cultures

Ramsden, M., Plant, L. D., Webster, N. J., Vaughan, P. F., Henderson, Z., and Pearson, H. A. (2001). Differential effects of unaggregated and aggregated amyloid beta protein (1-40) on K(+) channel currents in primary cultures of rat cerebellar granule and cortical neurones. J Neurochem 79, 699-712. 

Suspension cultures of Coffea arabica cv bourbon amarelho derived from primary callus cultures have been maintained on media (14) supplemented with cysteine, thiamine and kinetin (39). The cells were sieved through nylon nets of 1 mm mesh size to obtain small aggregates and single cells. Large aggregates were not used. 

Stably immobilized 3D flat aggregates and exhibited superior cell bioactivity with higher levels of liver-specific function maintenance in terms of albumin secretion, urea synthesis and cytochrome P-450 enzyme than 3D spheroid aggregates formed on GC films. These results suggested that the GC-based nanofibrous scaffolds could be useful for various applications such as bioartificial liver-assist devices and tissue engineering for liver regeneration as primary hepatocytes culture substrates. 

---