Aggregate-restraint

First, oleanolic acid (43) could induce the release of the prostaglandin I2 (PGI2) of bioactive prostanoid having smooth muscle relaxation action and platelet aggregation restraint action by the human coronary SMC (Smooth Muscle Cell). This effect was inhibited by a coxib-based nonsteroidal antiinflammatory drug celecoxib of a selective Cox-2 inhibitor that relieves pain and inflammation without harming the digestive tract. 

This approach can be used to give critical micellizatlpn concentrations as well as size distributions of aggregates — but is, of course, unable to distinguish between different forms of the association structures. In order to achieve this packing, restraints must be introduced. 

The MEKC behavior of surfactants of differing types versus SDS for the alkyl benzene homologous series is demonstrated in Figure 15.4. Because the data were normalized, slopes of one indicate similarity to SDS. As it can be observed from Figure 15.4, from all surfactants under consideration, only sodium deoxycholate (SDC, slope of 1.14 0.04) and LPFOS (slope of 0.696 + 0.009) exhibited distinctive behavior, despite the observations above concerning the restraints the solute set impart when phase selectivity is contrasted. The structure of SDC micellar aggregates is quite unusual being described by a helical model (78). The helix has the lateral surface covered by... 

The procedure allows three possible outputs (i) there is only one clustered structure that respects the initial restraints. In this case, a likely structure for the macromolecular complex has been found, (ii) There are multiple structures respecting initial restraints. Then, either there exist multiple native aggregation states, or the number of restraints used is insufficient to uniquely determine the structure of the complex. In any case, additional experiments adding new restraints should be performed, (iii) The algorithm cannot find structures satisfying the initial restraints. In this case, either the experimental data set is biased, or there is some issue in the interpretation of the experiment in terms of spatial restraints. 

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