Agarose reaction with epichlorohydrin

Reaction with epichlorohydrin-activated agarose Reaction with dextran cyclic imidocarbonate... 

Carbodi-imide-mediated reaction with epichlorohydrin-activated agarose previously treated with glycine Reaction with a syw-trichlorotriazine-activated polystyrene anion exchanger reaction with 1,4-benzoqumone-activated aminopropyl silica reaction with an N-hydroxysuccinimide ester derivative of 3-succinimidopropyl silica Carbodi-imide- or 2,4-toluenedi-isocyanate-mediated reaction with amino-or carboxy-aerosU... 

Carbodi-imide-mediated reaction with epichlorohydrin-activated agarose previously treated with glycine... 

Active immobilized forms of j3-amylase have been prepared by the reaction of the enzyme with agarose cyclic imidocarbonate and its derivatives, and by hydrophobic interaction with alkyl derivatives of agarose cross-linked with epichlorohydrin. ... 

Epoxyactivation of agarose beads. Wash the required amount of Sepharose CL-6B with 40 mL of distilled water/g of gel on a sintered funnel (see Note 7). Transfer the washed agarose to a 1 L conical flask and add 1 mL of distilled water/g of gel. To this moist gel, add 0.8 mL of NaOH/mL of gel and incubate the slurry for 1 h at 25 C on a rotary shaker. Raise the temperature to 34°C and add 1 mL of epichlorohydrin/mL of gel to the reaction mixture (21). Wash the epoxide-activated resin with 40 mL distilled water/g of gel on a grade 2 sintered funnel and use it directly for >diol activation (Fig. 4 Resin B). Determine the epoxy-content according to Note 7. 

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