Aflatest immunoaffinity column

Aflatoxins in Com, Raw Peanuts and Peanut Butter Immunoaffinity Column Method (Aflatest P column) Vicam 128... 

Another area where IAP is found to be beneficial is in the testing of afiatoxins. The development of Aflatest using IAP enrichment has allowed sample testing in minutes with limited laboratory facilities and without need for hazardous aflatoxin standards (135). An AOAC/IUPAC collaborative study was conducted to evaluate the effectiveness of the immunoaffinity column (Aflatest) for determination of aflatoxin. The method has been given official first action by the AOAC (128). 

Recently we completed an evaluation of the effectiveness of the Aflatest (Vicam) immunoaffinity column for analysis of corn, peanuts and peanut butter for aflatoxin (11). In this procedure the sample is extracted with Me0H/H20, filtered, and the extract diluted to <30% MeOH with water. An aliquot is then applied to the immunoaffinity column. The column is then washed with water and the aflatoxins eluted with methanol. Total aflatoxins are then determined by solution fluorometry with bromine (SFB), or individual toxins by reverse phase high performance liquid chromatography with post column I2 derivatization (PCD). In the collaborative study corn samples naturally contaminated with aflatoxins and samples of corn, peanuts and peanut butter spiked at 30, 20 and 10 ng/g, were analyzed by 24 collaborators. The results of this study are tabulated in Table V. 

Aflatest. This immunoaffinity column also met the lower detection requirement for the USFDA at a permissible limit of 20 ppb for aflatoxin. Qualitative results can be obtained using a florisil tip, however, the florisil tip was found difficult to interpret. Evaluation showed the quantitative analysis to be as accurate as other HPLC procedures, but much more rapid. Using the Aflatest for sample extract purification, and HPLC with post column derivatization using iodine, a limit of quantitation of 2.3 ppb for B and G is easily achieved. Sensitivity to B2 and G- is lower due to less specificity of the Aflatest antibodies to tnese toxins. 

Aflatoxins in corn, raw peanuts, and peanut butter - immunoaffinity column (Aflatest) method... 

---