Affinity important features

The third important feature distinguishing catecholamines from neu-ronally released norepinephrine involves epinephrine s affinity for -receptors. Norepinephrine has a very limited affinity for these receptors. Therefore, circulating epinephrine causes effects that differ from those of direct sympathetic innervation, including ... 

At least two enzymes compete for acetyl-CoA - the citrate synthase and 3-ke-tothiolase. The affinities of these enzymes differ for acetyl-CoA (Table l),and at low concentrations of it the citrate synthase reaction tends to dominate, provided that the concentration of 2/H/ is not inhibiting. The fine regulation of the citrate synthases of various poly(3HB) accumulating bacteria has been studied [ 14, 47, 48]. They appear to be controlled by cellular energy status indicators (ATP, NADH, NADPH) and/or intermediates of the TCA cycle. The 3-ketothio-lase has also been investigated [10-14,49, 50]. This enzyme is, above all, inhibited by CoASH [10,14,49]. This important feature will be further considered below. 

It is well known that a very important feature of many biological systems is specific recognition at the molecular level. Antibodies as a group are widely used for molecular recognition, e.g., affinity assays. This feature can be used by labeling an anti-human growth hormone antibody fraction with a fluorescent tag... 

The success of treating tumours, especially solid tumours, by systemic therapy depends on various characteristics of the tumour. Besides the importance of intrinsic drug activity and the potential targets within the tumour cells, drug pharmacokinetics and whole body distribution, site of delivery and the ability of site-specific targeting (affinity) are important features. 

In Lewis et al. s (225) QSAR study, eight CYP2C9 substrates where examined. In this case, it appeared as if the acid dissociation constant pXa, the compounds lipophilicity, and the number of hydrogen bond donor atoms are important features (Eq. 1, Table 8). These suggestions are supported by a good correlation with the binding affinity. 

One of the most important features in the immunosensor design is the proper choice of the immobilization method for keeping the affinity of the antibodies. As was previously demonstrated for Protein A, when the antibodies are immobilized through their Fc fragment to Protein A (or G), their Fab binding sites are mostly oriented away from the solid phase. As Protein A is able to link the Fc region of different antibodies, there is no need to modify the antibody with biotin. As an antecedent, we have previously demonstrated the utility of Protein A biocomposite (ProtA-GEB) for the universal attachment of antibodies with different specificities [54]. 

The fundamental criteria are the affinity sequences found for the complexes formed in aqueous solution by various groups of ligand atoms. These sequences turn out to have very important features in common for the halide, chalcogen and nitrogen groups. For acceptors termed (a) and (b), respectively, they are as follows ... 

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