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Advanced Techniques of Separation and Purification

The various types of Chromatographic Separation have been developed partly to avoid the above disadvantages, but (more particularly) to provide methods of separation on a micro-scale. Three methods are described below  [Pg.48]

Paper chromatography in particular frequently enables the components of a mixture to be separated and identified when only 1-2 mg. of the mixture are available, the process being independent of the relative solubilities of the components. [Pg.48]

A mixture can often be separated into its components by utilising their selective adsorption from solution by a suitable substance, such as active alumina the separation can be readily followed if the components are coloured. [Pg.48]

If the components are colourless, their separation can often be followed by working in a quartz (or special glass) tube which is placed in the light of a mercury lamp. The separate zones are then often revealed by their fluorescence. [Pg.49]

The process of chromatographic separation is illustrated in the following experiment, in which a wider tube than usual is employed to give a reasonably rapid separation within the time normally available to students. The alumina employed is the usual active alumina as supplied by dealers. [Pg.49]


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