Acyl-CoA alcohol transacylase

Khan A A, Kolattukudy P E 1975 Solubilization of fatty acid synthetase, acyl-CoA reductase, and fatty acyl-CoA alcohol transacylase from the microsomes of Euglena gracilis. Arch Biochem Biophys 170 400-408... 

FIGURE 4.5 Reaction scheme for conversion of octanoic acid into esters, E, acyl-thiokinase E2, aeyl-CoA-alcohol-transacylase E3, acyl-CoA reductase E4, alcohol-NAD oxydoreductase. (From Tressl, R., R Drawert, J. Agric. Food Chem., 21, p. 560, 1973. With permission.)... 

European Atomic Energy Community Ethylene-vinyl alcohol Malonyl-CoA acyl carrier protein Malonyl-CoA-ACP transacylase 3-Ketoacyl-CoA reductase... 

Wax ester biosynthesis probably involves an acyl transfer mechanism. The high thioesterase activity found in crude plant extracts makes it difficult to demonstrate acyl-CoA involvement in wax ester synthesis. However, partial purification of an acetone powder extract from the leaves of B. oleracea gave a protein fraction that catalyzed an acyl-CoA-dependent esterification of fatty alcohols (222). Additionally the acetone powder extract from B. oleracea leaves appeared to catalyze the direct transfer of acyl moieties from phospholipids to fatty alcohols. The leaf extract also catalyzed under appropriate conditions the esterification of fatty alcohols to free fatty acids. The transacylase mechanism is likely to be the main mechanism of wax ester synthesis in vivo. The fact that labeled wax esters were synthesized by a membrane-bound microsomal fraction from Hordeum vulgare leaves following incubation with radioactive alcohols, but not after incubation with free fatty acids (17), is consistent with the proposed acyl transfer mechanism. In E. gracilis the acyl-CoA reductase is functionally coupled to the acyl transferase (227). Both of these activities were solubilized from the microsomes... 

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