Activity-based protein profiling ABPP site-directed

Activity-based protein profiling (ABPP) is a chemical proteomic strategy in which active-site-directed covalent probes are used to profile the functional states of enzymes in complex proteomes. Activity-based probes (ABPs) can distinguish active enzymes from their inactive zymogens or inhibitor-bound forms. They contain a reactive group intended to modify enzyme active sites covalently and a reporter group (typically rhodamine or biotin) that assists in detection and identification of protein targets. 

Another area in chemical biology that the triazole-forming click reactions have revolutionized is activity-based protein profiling (ABPP). ABPP is a chemical biology platform that uses active site-directed probes [61] to study the dymamic expression and function of mechanistically similar enzymes in their various... 

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