Activity amplification

This section will demonstrate the first sergeants and soldiers-type helix command surface experiment, in which thermo-driven chiroptical transfer and amplification in optically inactive polysilane film from grafted (or spin-coated) optically active helical polysilane onto quartz substrate [92]. Although helix and optical activity amplification phenomena based on the sergeants and soldiers principle was mainly investigated in polymer stereochemistry, the orientation and physical properties of a thick layer deposited onto a solid surface and controlled by a monolayer command film based on command surface principles was established in photochemical material and surface science [93,94]. Both sergeants and soldiers and command surface experiments appear to have been developed independently. 

Tiazofurin and selenazofurin metabolites bind to different sites on ionosine monophosphate dehydrogenase to synergistically inhibit its activity Amplification of hepatotoxicity of chlordecone-I-CCI4 by inhibition of hepatocellular repair due to calcium flooding and depletion of cellular energy... 

The development of various EIA procedures has led to confusing terminology and classifications, which are often misleading concerning their fundamental features and tend to obscure their relative merits. Not surprisingly, many comparative studies produced inconclusive results. The frequent comparison of the relative merits of radioactive and enzyme labels, as evaluated in RIA by saturation analysis and in ELISA by immunometric analysis, is basically faulty since the underlying principles of these assays are different (Ekins, 1980). Here, EIA will be classified according to their essential differences to expose the inherent limitations and potentials of each assay EIA are based either on Activity Amplification (AA) or Activity Modulation (AM). 

Sensitivity and detectability of activity amplification and activity modulation assays... 

Practical implications for activity amplification (AA) and activity modulation (AM)... 

Activity amplification assays Activity modulation assays Immunohistochemistry... 

Processing of data and reporting of results of activity-amplification assays... 

Figure 5. Alternative pathway of complement activation amplification loop. Schematic outline of the alternative pathway and the positive feedback loop initiated by C3b. Because of the uncertainty over whether all activating substances utilize the same initial steps, these steps have not been included.

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