Active wash

Aldridge has shown that the addition of eserine to intact erythrocytes causes a fall of cholinesterase activity to 20 per cent, and that the activity returns when the cells are washed at 0°. With dimethyl p-nitrophenyl phosphate there is a similar fall of activity, but washing at 0° does not restore the activity. Washing at 37°, however, is effective. 

While keeping the principle of washing as a dilution, the chemist would search for a solution to get an immediate and complete neutralization. This is the actual goal of searching for solutions of active wash. 

Time elapsed before starting active washing... 

Nafion-H has also been shown to be active in the Ritter reaction. Treatment of alcohols, such as benzyl alcohols, 1-adamantanol [Eq. (5.346)], and 2-norborneols with acetonitrile or benzonitrile in the presence of Nafion-H under forcing conditions (140-145°C, 18—48h), affords the corresponding carboxamides in moderate to excellent yields (40-99%).652 Nafion-H can be reused after a simple activation (washing with deionized water and acetone followed by drying at 105°C). 

Distribution of D-pantolactone-specific lactonase is narrow only filamentous fungi of three genera, i.e., Fusarium, Gibberella and Cylindrocarpon, show high hydrolytic activity. Washed cells of these fungi, as well as the purified lactonase, catalyze well this stereospecific hydrolysis. For example, D-pantolactone in a racemic mixture of pantolactone (700 mg/ml) was almost... 

Removal of by-products from activated wash on funnel polysaccharide dialysis (alternatively organic solvent precipitation or gel chromatography)... 

In contrast to radioactively labeled DNA probes, the medium for prehybridization and hybridization is adapted to RNA (2) mainly to prevent contamination by RNases. However, diethyl pyrocarbonate (DEPC) treatment of the water is not necessary. Good quality distilled water can be used instead to prepare the solutions as some contain SDS, which inhibits RNase activity (washing solutions) whereas others are kept as highly concentrated stock solutions (20X SSC), are autoclaved and stored at 4°C (TN buffer, alkaline TNM buffer, and TE buffer), or are made freshly (SSC dilutions, blocking buffer, ABC solution, and color solution). 

With chemical absorption, the reaction rate increases with increasing temperature. Chemically active wash liquids are characterized by high selectivities and good load capacities even under low absorbate concentrations in the gas phase. 

Figure 30 Swelling test of a collagen film. Water uptake for formulations A and B (see Table 7) at 1% active washing substance for both, in the presence of different amounts of nonhydrolyzed wheat proteins. Solutions were prepared in phosphate buffer 1 mM pH 6.0. Incubation was made at 50°C for 24 hr.

NORIT SX IG Steam activated washed powder DARCO KBB Chemical activated powder... 

Another brochure named Dossier Entreprise-Hopital prise en charge des projections chimiques (Chemical splashes from Factory to Hospital), written by emergency physicians, ophthalmologists, dermatologists, occupational physicians, and chemists explains the specificity of chenucal skin lesions and the interest and the use of active washing after chemical splashes [269]. 

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