Action on enzymes including fibrinolysin

The close relationship of the chief proteolytic enzyme system of plasma, the plasminogen system, to coagulation changes has led to many studies on the relationship of heparin to fibrinolysin. Heparin does not inhibit plasmin, or the fibrinolytic activity of Aspergillus proteus, but inhibits various fibrinolytic activators directly . For antifibrinolytic activity, heparin requires a co-factor found only in plasma, and this may be the heparin cofactor itself. Heparin therefore may lack antifibrinolytic activity on a purified system due to the lack of this factor . Buluk and Januszko report that fibrinolysis of 

While heparin increases the temperature stability of trypsin and thrombin, it increases the instability of /3-amylase, even though it shows no immediate inhibition. A slow reaction occurs leading to irreversible inactivation of amylase, probably due to salt formation followed by denaturation of the protein with the formation of higher complexes . Heparin has no effect on the serum or plasma amylase activities . 

Heparin, de-esterified nitrated hyaluronic acid, the related sulphonic acid dyes (e.g. chlorazol fast pink) and a non-specific inhibitor of rabbit and human serum, all inhibit various hyaluronidases from different sources but there are great differences, depending on substrate, enzyme and inhibitor. As inhibitors, cellulose trisulphuric acid and chitin disulphuric acid are much stronger, while treburon is similar to heparin. 

Heparin, liquoid, farma , congo red, trypan blue , and treburon inhibit egg-white lysozyme. In fact, heparin and lysozyme mutually inhibit each other in vitro and even in vivo, as prolonged treatment with heparin results in reduction of salivary lysozyme values. 

Pancreatic ribonuclease is markedly inhibited by heparinoids but not by mucopolysaccharides, with sulphated polyvinyl alcohol sulphated corn amylosOsulphated cellulose sulphated corn amylopectin sulphated dextran, sulphated pectic acid, polyvinyl sulphonate with 2 T 7 per cent sulphur sulphated nitro chitin, sulphated nitro chitosan and heparin . Single injections of heparin in mice result in significant inhibition of the acid alkaline ribonuclease of the liver after the injection. This probably explains the accumulation of ribonucleic acid in tissue culture cells in the presence of heparin. Heparin also inhibits rat acid phosphatase, glucuronidase , catalase , fumarase and elastase. 

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