Acridine orange labeling

Acridine orange (AO) Alkaline cell green Acid cell red/purple 502 526 Cell acidity 10.0 33 pL Not used in yeast/protein mixtures. Slightly labels the membrane green... 

Protocol 12.1 Dissection Techniques, 203 Protocol 12.2 Antibody Staining, 207 Protocol 12.3 (3-calactosidase Detection, 212 Protocol 12.4 BrdU Labeling, 215 Protocol 12.5 In Situ Hybridization, 216 Protocol 12.6 Cobalt Sulfide, 220 Protocol 12.7 Acridine Orange, 221 Protocol 12.8 TUNEL Staining, 222 Protocol 12.9 Phalloidin, 224 Protocol 12.10 DAPI, 225... 

Although more labor intensive than the acridine orange method, the TUNEL-labeling technique (terminal deoxynucleotidyl transferase [TdT]-mediated dUTP nick end labeling) provides a permanent record for labeling dead cells. (From Gavrieli et al. 1992 White et al. 1996 protocol and additional modifications were provided by P. Kurada and Kristen White.)... 

Nucleic acids do not display the same promiscuous chemical reactivity of proteins. Instead, individual synthetic nucleotides can display a unique functional group that can be exploited even more for direct attachment of probes (117). The simplest method of labeling DNA uses high-affinity bis-intercalating dyes such as ethidium bromide, acridine, and thiazole orange monomers... 

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