Acid phosphatase combined effects

Coles and Gross (5, 6) have found that some of the penicillinase of Staphylococcus aureus is bound ionically to the surface of the cell wall and can be liberated by low concentrations of anions. Polyanions that do not penetrate the cell wall and tricarboxylic acids were particularly effective as release agents Weimberg and Orton (16) had found earlier that acid phosphatase could be released from the cell surface of Saccharo-myces mellis by chloride ions in combination with 2-mercaptoethanol and concluded that this enzyme is held on the cell surface by a combination of electrostatic forces and disulfide bonds. Likewise, polygalacturonase is released from the surface of spores of Geotrichum candidum with polyanions and 2-mercaptoethanol (2). 

Thiebart-Fassey, I. and Hervagault, j.-F. (1993) Combined effects of diffusional hindrances, electrostatic repulsion and product inhibition on the kinetic properties of a bound acid phosphatase. FEBS Letters 334, 89-94. 

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