Accuracy evaluation section preparation

The most recent bioanalytical Workshop Report (Viswanathan 2007) devotes considerable space to this topic and some recommendations not discussed previously (Section 9.4.7b) are included below. There should be some assessment of both reproducibility and accuracy of the reported concentration. Sufficient data should be generated to demonstrate that the current matrix (i.e. the incurred sample matrix) produces results similar to those previously validated. It is recognized that accuracy of the result generated from incurred samples can be more difficult to assess. It requires evaluation of any additional factors besides reproducibility upon storage, which could perturb the reported concentration. These could include metabolites converted to parent during sample preparation or LC-MS/MS analysis, matrix effects from high concentrations of metabolites, or variable recovery between analyte and internal standard (Viswanathan 2007). Most of these phenomena are those described previously (Jemal 2002) and discussed in Section 9.4.7b. 

In summary, the utility of micro-SERS spectroscopy for the evaluation of potential-dependent interfacial com-petititve and displacement reactions at chargwl surface has been demonstrated. The data obtained allow the determination of the chemical identity, structure, orientation, competitive and displacement adsorption of cationic surfactants and nitrophenol in the first adsorption layer. The examples of these measurements in the field of surfactants and organic pollutants reviewed in this article were selected to illustrate the sensitivity, molecular specificity of adsorption processes, accuracy, ease of substrate preparation, and manifold applications of Raman analysis. The spatial resolution of the laser microprobe, coupled with the 10 enhancement of the Raman cross-section, means that picogram quantities of material localized to pm-sized surfaces areas can be detected and identified by SERS vibrational spectroscopy. 

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