Abbott Molecular

The held of PCR/ESI-MS is currently dominated by Abbott Molecular (Abbott Park, IL) their PLEX-ID platform contains an in-house database that is crucial for data analysis. As a recommended protocol for this type of work, the hrst step is extraction of DNA from the mixture to be analyzed. Many different types of extraction kits exist and are suitable for PCR-MS analysis however, most published studies have used the Qiagen DNA extraction kits (Qiagen, Valencia, CA) and KingFisher extraction (Thermo Scientihc, Waltham, MA). The resulting DNA is then aliquoted into a 96-well plate for PCR, where each well contains a single set of broad-range primers to amplify the DNA present. 

Dallas, G. and Abbott, S. D., New approaches to the analysis of low-molecular-weight polymers, in Liquid Chromatographic Analysis of Food and Beverages, Vol. 2, Charalambous, G., Ed., Academic Press, New York, 1979, 509. 

Ray, C., Abbott, A.G. and Hussey, R.S. (1994) Trans-splicing of a Meloidogyne incognita mRNA encoding a putative esophageal gland protein. Molecular and Biochemical Parasitology 68, 93—101. 

Fig. 10. A. Acetic acid-urea-triton-X-100 polyacrylamide gel electrophoresis [15] of the histones used to reconstitute 208-12 nucleosome arrays consisting of recombinant H2A.Z (lane 2) or recombinant H2A.1 (lane 3). Lanes 1 and 4 respectively are chicken erythrocyte and calf thymus histones used as markers [42]. B. Ionic strength (NaCl concentration) dependence of the average sedimentation coelRcient (s2o,w) of reconstituted 208-12 nucleosome arrays containing either H2A.1 (O) or H2A.Z ( ) [42]. The dotted line represents the behavior of a 208-12 complex reconstituted with chicken erythrocyte histones [406]. [Reproduced from Abbott D.W. et al. (2001) I. Biol. Chem. 276, 41945-41949, with permission from The American Society for Biochemistry and Molecular Biology.]...

Abbott, M. T., Udenfriend, S-, in Molecular mechanisms of oxygen activation. 

An interesting detection system which needs no separation step and is amenable to automation is the Abbott TDx analyzer. It is based on measurement of increase in polarization of the fluorescence of a small fluorescent labeled antigen when bound by a larger molecular weight antibody. It has been used for detection of contaminants, especially smaller antigens like drugs and steroids in food (76). Its detection range is pg/ml which can be... 

MT Abbott, S Udenfriend. cx-Ketoglutarate-coupled dioxygenases. In O Hayashi, ed. Molecular Mechanisms of Oxygen Activation. New York Academic Press, 1974, pp 167-214. 

The combination or tethering of molecular fragments that were derived from known drugs or lead structures with known and desired bioactivity often results in chimera that also exhibit the desired function. Linking of some few active fragments can result in multiple lead structures, as shown recently by researchers at Abbott Laboratories. ... 

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