Antibodies molecular weight

Data of essentially this sort, obtained with arsanilic acid as the hapten and the rabbit as the experimental animal, have been published by Haurowitz and his collaborators in a paper reporting many interesting experiments. The equivalent weight of antibody p arsanilic acid residue was found to vary from 23,000 for antigens with very many attached haptens to 51,000 for those with only a few. The expected value for saturation of all haptens by bivalent antibody molecules is one-half the antibody molecular weight, that is, about 79,000. The low experimental values are probably due to failure of some haptens to combine with antibodies. In particular, if two haptens are attached to the same tyrosine or histidine residue steric interactions of antibodies may permit only one of the haptens to be effective. ... 

As a low molecular weight compound DAS is not significantly immunogenic, ie, it is a hapten and thus requires conjugation to a suitable antigenic carrier in order to eUcit a successfiil antibody response in animals. DAS treated with succinic anhydride results in the D AS—hemisuccinate (DAS—HMS) shown. 

Sepharose (e.g. Sepharose CL and Bio-Gel A) is a bead form of agarose gel which is useful for the fractionation of high molecular weight substances, for molecular weight determinations of large molecules (molecular weight > 5000), and for the immobilisation of enzymes, antibodies, hormones and receptors usually for affinity chromatography applications. 

Lactoferrin resembles transferrin in terms of molecular weight, amino-acid sequence homology and number of Fe(lII) binding sites. Lactoferrin is released from activated PMNs upon degranulation and may play a role in myelopoiesis, primary antibody response, lymphocyte proliferation, cytokine production and complement activation. 

Heparin-induced thrombocytopenia A clinical syndrome of IgG antibody production against the heparin-platelet factor 4 complex occurring in approximately 1% to 5% of patients exposed to either heparin or low-molecular-weight heparin. Heparin-induced thrombocytopenia results in excess production of thrombin, platelet aggregation, and thrombocytopenia (due to platelet clumping), often leading to venous and arterial thrombosis, amputation of extremities, and death. 

---