A-Morpholino

AACC Method32-07for TDF, SDF, and IDF. This approved method is a modification of the Prosky method. The phosphate buffer is replaced by a buffer of 2(A[-morpholino)ethanesulfonic acid (MES) [4432-31-9] and tris(hydroxymethyl)aminomethane (TRIS) [77-86-1]. Precision is improved and analysis time is reduced. I DE may be determined on a separate sample or calculated by summing the SDE and IDE values. The MES /TRIS-modified method has received final approval by AACC and official first action by AO AC (5). 

Figure 2 Stability of /3-poly(L-malate) measured by its activity to inhibit purified DNA polymerase a of P. polyceph-alum. The relative degree of inhibition is shown (100 rel. units refer to complete inhibition). The DNA polymerase assay was carried out in the presence of 5 /tg/ml /S-poly(L-malate) as described [4]. The polymer was preincubated for 7 days at 4°C in the following buffer solutions (50 mM) KCl/HCl (—A—). Citrate (—V—). 2-(A/-Morpholino)-ethanesulfonic acid, sodium salt (—O—). Sodium phosphate (— —). N-(2-Hydroxyethyl)piperazine-N -(2-ethanesul-fonic acid), sodium salt (— — ). N,N-b s (2-Hydroxyethyl)-glycine, sodium salt (—T—). Tris/HCl (— —). 3-(Cyclo-hexylamino)-l-propanesulfonic acid, sodium salt (— —).

A benzotriazole-assisted synthesis of ethyl 5-aminoimidazo[2,l-h]thia-zole-6-carboxylate 65 has been developed (96TL1787). Traces of compound 63 were isolated from 2-aminothiazole 23 using benzotriazole and freshly distilled ethyl glyoxylate in benzene, whereas by application of a-benzotria-zolyl-a-morpholino acetate with methyl iodide in THF, 63 was isolated in 40% yield. Subsequent treatment of 63 with potassium cyanide in ethanol at rt involves substitution of the benzotriazole moiety. Intramolecular cycli-zation of 64 yields 65, albeit in 10% yield. a-Ketoester 66 is also obtained (Scheme 3). 

If the vinyleyclobutanone is substituted in the 3-position by a morpholino group, which can stabilize a cationic center, then C3 migrates instead of the carbonyl group on treatment with /Moluenesulfonic acid to give 3.70... 

SB medium (SB) 30 g/L Bacto-tryptone (Difco, Detroit, MI), 20 g/L yeast extract (Difco), 10 g/L MOPS (3-[A/-morpholino]propanesulfonic acid), pH 7 0, containing 10 pg/mL tetracycline (Boehringer Mannheim, Indianapolis, IN)... 

Reagents. Unless noted, all reagents are commonly available from a number of sources. The following buffers and solutions are needed for kinase screening MES buffer 30 mM 2-(A-morpholino)ethanesulfonic acid (MES), 10 mM MgCl2, and 0.4 mg/ml bovine serum albumin (BSA) pH... 

Holmes et al. (1998) performed two enzymatic reactions, the lipase-catalyzed hydrolysis of y>-nitrophenol butyrate and lipoxygenase-catalyzed peroxidation of linoleic acid, in w/c microemulsions stabilized by a fluorinated two-chained sulfosuccinate surfactant (di-HCF4). The activity of both enzymes in the w/c microemulsion environment was found to be essentially equivalent to that in a water/heptane microemulsion stabilized by Aerosol OT, a surfactant with the same headgroup as di-HCF4. The buffer 2-(A-morpholino)ethanesulfonic acid (MES) was used to fix the pH in the range 5-6. 

Seeds contained in the tip of a spatula were homogeneously dispersed in a Petri dish.(55mm 0) on Whatman GF/A paper. For preconditioning, 1 mL of a solution of 0.3 mM of 2-[A/-Morpholino]ethanesulfonic acid was added to the filter,... 

Hydroxythiadiazoles exhibit marked acid properties (see Section 4.26.2.8) and exist essentially in the hydroxy form (68AHC(9)107). Alkylation with alkyl halides or sulfates and base in dipolar aprotic solvents usually forms ethers. In media where the oxygen atom is screened by solvation or by formation of ion pairs, mixtures of N- and O -alkylation products are formed. The reaction of 3-chloro-4-hydroxy-l,2,5-thiadiazole with epichlorohydrin catalyzed by piperidine in the absence of a solvent gave a mixture of N- and O-alkylated products in about equal amounts (72JMC651). Treatment of 3 - hydroxy-4-(A - morpholino) -... 

Four moles of periodate were required for the oxidation of 1-deoxy-l-piperidino-D-fructose and I-deoxy-I-morpholino-D-fructose at pH 4.5. As indicated, one mole of these compounds would be expected to consume four moles of oxidant to produce a-piperidino- or a-morpholino-acetic acid, three moles of formic acid, and one mole of formaldehyde. Although the acid titration showed that three moles of acid were formed, the presence... 

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