A-Methyl mannose

Figure 7 Quantitative inhibition assays in oligosaccharide microarray. (A) Determination of the concentration of soluble a-methyl mannose to inhibit 50% of Con A binding to spotted mannose. (B) Determination of the concentration of a-methyl mannose to inhibit 50% of Con A binding to spotted glucose. Each data point represents the meaniSD for 10 spots from 2 independent experiments.

Con A Jack bean seeds a-D-Mannose, a-D-glucose a-D-Methyl mannose... 

Anti-a-D-mannose antibodies have been isolated from the serum of rabbits immunized with the glycoconjugate of a-D-mannose and bovine serum albumin [85], The antibodies were purified by affinity chromatography with adsorption on a mannosyl-Sepharose column and elution with a-D-mannose or methyl a-D-mannoside. Such antibodies should be especially useful for studying the detection of diseases due to the appearance of abnormal glycoproteins that contain mannose polymers. 

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