5.8S rDNA

Chilton, N.L.B., Hoste, H., Hung, G.-C., Beveridge, I. and Gasser, R.B. (1997b) The 5.8S rDNA sequences of 18 species of bursate nematodes (order Strongylida) comparison with Rhabditid and Tylenchid nematodes. International Journal for Parasitology 27, 119-124. 

Penna, A., Vila, M., Fraga, S., Giacobbe, M.G., Andreoni, E, Riobo, P., and Vemesi, C. 2005. Characterization of Ostreopsis and Coolia (Dinophyceae) isolates in the western Mediterranean sea based on morphology, toxicity and internal h an-scribed spacer 5.8S rDNA sequences. Journal ofPhycology A y.2 2—225. 

The systemic nature of C. phalaridis is not typical of Claviceps. Walker (1970) and Uecker (1980) suggested C. phalaridis should be considered a Balansia, although the systemic nature of infection would be more characteristic of Epichloe than Balansia. However, phylogenetic analysis of 5.8S rDNA and ITS1 and ITS2 spacers by Pazoutova (2001) clearly place it within Claviceps. 

Our study (Pazoutova et al., 2000) established the population structure of C. purpurea and characterized the groups and isolates by host or habitat preferences, phenotypic traits used in previous studies (conidial morphology, alkaloid type, properties of sclerotia), as well as by DNA analysis (using RAPD and EcoRI restriction site polymorphism in the 5.8S rDNA). Thus, the ambiguous, even contradictory groupings found by previous researchers and based on only one or two characters were incorporated into one system. Three groups were identified ... 

De Jonckheere, J.F. (1998). Sequence variation in the ribosomal internal transcribed spacers, including the 5.8S rDNA, of Naegleria spp. Protist 149, 221-228. 

The main dataset (dataset 1) included 144 terminals (136 species), representing part of 25S rDNA (20 bp), ITSl (220-316 bp), parts of 5.8S rDNA (87-96 bp), ITS2 (245-294 bp), part of fmL intron (230-296 bp), 3-exon tmL (51-52 bp), tmL-tmP spacer (41-66 bp), and part of tmP (13-22 bp). For nine species of these 144 terminals ITS 1 or TTSl +ITS2 data were not available. Preliminary... 

C. purpurea isolates were analyzed using RAPD and AFLP (Pazoutova et al., 2000 Pazoutova et al., 2002). The difference between populations were considerable, as both methods detected only 1-3 bands shared between populations. Together with comparison of rDNA (ITS1-5.8S-ITS2 region), all three methods suggest that the Gl population is ancestral to the G3 and G2, which are more homogenous. 

Polymerase chain reaction (PCR)-based methods PCR (specific primers) PCR-RFLP of the 5.8s ITS rDNA region Species Species... 

Figure 5. Alignment of sequence data of rDNA from chironomid species. Sequence data is aligned showing similarities and differences. (5A) Alignment of C. tentans (Chironomus tentans), D. fumidus (Dicrotendipes fumidus,), and C, bicinctus (Cricotopus hicinctus) from the 18S subunit of rDNA. (5B) Alignment ofpartial /8S, ITS-], 5.8S, ITS-2, and 2 8S subunits of rDNA from 14 chironomid species C. tentans (Chironomus tentans) Genbank accession X992I2, D.fumidus (Dicrotendipes fumidus,) Genbank accession, ...

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