Zone Electrophoresis of Polysaccharides

Rienits later studied the zone-electrophoretic behavior of hyaluronic acid, chondroitin hydrogen sulfate, and heparin on paper strips in nonborate buffers he found that, whereas hyaluronic acid can be separated from chondroitin hydrogen sulfate and heparin, the latter two mucopolysaccharides cannot be separated. The mucopolysaccharides could be located by the use of a method similar to that of Gardell, Gordon and Aqvist, or by staining the pherogram with Toluidine Blue. The mobility of hyalu- 

Kent and coworkers briefly reported the detection, after application of zone electrophoresis in barbiturate and other buffers, of a number of neutral polysaccharides, although no details of mobilities were noted. In a borate buffer at pH 8.0, amylopectin was observed to migrate toward the cathode and to be stained red with iodine, whereas amylose is stained blue and remains stationary. 

Preece and Hobkirk have attempted the fractionation of water-soluble, cereal polysaccharides (obtained from rye and oats), using zone electrophoresis on paper in a borate buffer at pH 11 and an acetate buffer at pH 4. The fractions were located on the pherogram by segmenting it, eluting 

Cereal Buffer Movement Glucos Xylos Galactos Arabinos  

In a detailed study of the zone-electrophoretic behavior of amylosac-charides on paper using a borate buffer (pH 10), Foster, Newton-Heam and Stacey showed that, whilst the amylopectin (blue value, 0.18) underwent appreciable migration toward the anode with but little adsorption of the polysaccharide in the path of movement, the pattern of movement of amylose (blue value, 1.35) depended on the amoimt of the polysaccharide put on the paper. Thus, a sufficiently small amount was adsorbed at the origin, but, with larger amounts, migration toward the anode occurred. A mixture of amylose and amylopectin was easily resolved, whereas, with 

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