Zero-length crosslinking agents

Carbodiimides are zero length crosslinking agents which mediate amide bond formation between carboxylie aeid groups and amino groups present in the protein. The crosslinking reaction can be intramolecular or intermolecular. Intramolecular crosslinking is often used... 

Carbodiimides are often used in the crosslinking of proteins. They are a family of zero length protein crosslinking agents, which promote the formation of covalent crosslinks between reactive side groups of amino acids, but do not remain as part of the crosslinks. 

Fig. 11 Gel concentration dependence of the diffusion constant (O) and persistent length ) of the slow mode of chemically crosslinked gelatin gel [35] ( and are the diffusion coefficient and persistent length when the concentration of the crosslinking agent is zero).

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