Zebrafish, transgenesis

Grabber C, Joly JS, Wittbrodt, J. (2004b) Highly efficient zebrafish transgenesis mediated by the meganuclease 1-Scel. Methods Cell Biol in press. 

Mosimann C, Zon LI (2011) Advanced zebrafish transgenesis with Tol2 and application for Cre/lox recombination experiments. Methods CeU Biol 104 173-194... 

The medaka (Oryzias latipes) is a teleost native to Japan, Korea, China, and Taiwan23. Similar to the zebrafish, it produces transparent embryos that allow easy observation and manipulation. Medaka is a popular model organism in Japan and the first fish species in which stable transgenesis was established, it provides some complimentary features to the zebrafish such as adaptation to a wide range of temperatures.22 The identification of sex specific markers, secondary sex characters and a sex determination gene have lead to increasing attraction of the medaka as a model for research on sex determination and differentiation23-28 (photo Andre Kunzelmann, UFZ). 

Katoh K, Koshimoto H, Tani A, Mori K (1993) Coding of odor molecules by mitral/tufted cells in rabbit olfactory bulb. II. Aromatic compounds. J Neurophysiol 70 2161-2175 Kawakami K (2004) Transgenesis and gene trap methods in zebrafish by using the Tol2 transpos-able element. Methods Cell Biol 77 201-222... 

Recent Advances in Meganuclease-and Transposon-Mediated Transgenesis of Medaka and Zebrafish... 

Westerfield M (1995) The zebrafish book. University of Oregon Press, Eugene. Yamamoto T (1975) Medaka (killifish), biology and strains. Keigaku, Tokyo. Meng A, lessen JR, Lin S (1999) Transgenesis. Methods Cell Biol 60 133-148. Hyatt TM, Ekker SC (1999) Vectors and techniques for ectopic gene expression in zebrafish. Methods Cell Biol 59 117-126. 

Key words Zebrafish, Gluconeogenesis, Diabetes, Metabolic disease. Fasting metabolism. Small-molecule screening, Bioliuninescence, Phosphoenolpyruvate-carboxykinase, pckl. Transgenesis... 

---