Zebrafish embryos microinjection

Wang W, Liu X, Gelinas D, Ciruna B, Sun Y (2007) A fully automated robotic system for microinjection of Zebrafish embryos. PLoS ONE 2(9) e862... 

Mizell, M. and E.S. Romig. The aquatic vertebrate embryo as a sentinel for toxins zebrafish embryo dechorionation and perivitelline space microinjection. Int. J. Dev. Biol. 41 411-423, 1997. 

Fig. 1 Microinjection of zebrafish embryos, (a) A typical setup for microinjection. (b) Recently fertilized eggs. The insets show early left) and late right) phases of one-cell-stage embryos, (c) Microinjection plate an acrylic plate with V-shaped grooves for holding embryos, (d) One-cell-stage embryos are arrayed in the grooves for microinjection. Scale bar, 2 mm. (e) DNA constmcts are injected into an embryo by penetrating the blastomere with the micropipette through the chorion. Scale bar, 200 pm...

The first transgene to be delivered into medaka embryos was the 6-crystalline gene of chicken (10) resulting in transient mosaic expression of the transgene. In 1988, Stuart McMurray, and Westerfield reported the first successful germline transmission and subsequent expression of a transgene on microinjection in a teleost genetic model system (zebrafish (12)). 

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