Yeasts thermally denatured

Because of their relatively low molecular weight (70 to 90 nucleotide residues), transfer ribonucleic acids are of special interest for 13C NMR investigations [769, 778, 782-784] of nucleic acids. Using a tube of 20 mm o.d., a sample of thermally denatured yeast... 

In other 13C studies of raffinose, stachyose,170 various vitamin B12 derivatives,171 and the anomeric equilibria of D-fructose and D-turanose in aqueous solution,172 partially relaxed, Fourier-transform spectra, and the values of obtained from them, have been elegantly used to confirm the spectral assignments. The T, values of the 13C nuclei of thermally denatured, yeast fRNA indicate that its D-ribose phosphate backbone undergoes rapid segmental motion at 81°. In contrast, the values of Tx for folded fRNA yielded no evidence of segmental motion.1720... 

Figure 4. Effect of 1 M urea on endotherm of yeast alcohol dehydrogenase. Addition of urea results in the elimination of the low temperature transition (bottom) as compared to the scan in the absence of urea (top). A low temperature exotherm is also observed which can be attributed to the aggregation of the thermally denatured subunit...

Galisteo M L, Mateo P L, Sanchez-Ruiz J M (1991). Kinetic study on the irreversible thermal denaturation of yeast phosphoglycerate kinase. Biochem. 30(8) 2061-2066. 

Brewer, J. M., and Wampler, J. E. (2001) A differential scanning calorimetric study of the effects of metal ions, substrate/ product, substrate analogues and chaotropac anions on the thermal denaturation of yeast enolase 1, Int. J. Biol. Macromol. Vol. 28, p>p. 213-218. 

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