Yeast cells scanning electron micrograph

A FIGURE 21-11 The fission yeast S. pombe. (a) Scanning electron micrograph of S. pombe cells at various stages of the cell cycle. Long cells are about to enter mitosis short cells have just passed through cytokinesis, (b) Main events In the... 

Nikolova and Ward [72,73] studied production of phenylacetyl carbinol from benz-aldehyde and pyruvate by whole-cell yeast biotransformation in two-phase systems. For the biocatalyst preparation fresh pressed commercial baker s yeast (50 g) was suspended in 50 ml 0.05 M sodium citrate buffer (pH 6.0) and lyophilized. Aliquots of 300 mg of lyophilized cells were mixed witii 1 g celite and the mixture was resuspended in 0.05 M sodium citrate buffer (pH 6.0). The suspension was lyophilized again and stored at 4°C. Scanning electron micrographs of the carrier celite and yeast cells lyophilized on celite are given in Fig. 1. Prior to use, organic solvents purchased in anhydrous form were saturated with 0,05 M sodium citrate buffer (pH 6.0). The same buffer was used as an aqueous component of the biphasic systems. 

Figure 1 Scanning electron micrograph of the celite carrier (left) and yeast cells adsorbed to celite (right). (From Ref. 73.)...

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