Yeast cells cytoplasm

Fig. 2.7 Germ tube formation by Candida albicans. For simplicity the diagram merely illustrates the nuclear content of the parental yeast cell and the developing germ tube. In real life there is a complex rearrangement of cytoplasmic constituents which results in all parts except the apex becoming highly vacuolated.

Figure 12.2 Copper chaperone function, (a) Copper homeostasis in Enterococcus hirae is affected by the proteins encoded by the cop operon. CopA, Cu1+-import ATPase CopB, Cu1+-export ATPase CopY, Cu1+-responsive repressor copZ, chaperone for Cu1+ delivery to CopY. (b) The CTR family of proteins transports copper into yeast cells. Atxlp delivers copper to the CPx-type ATPases located in the post Golgi apparatus for the maturation of Fet3p. (c) Coxl7p delivers copper to the mitochondrial intermembrane space for incorporation into cytochrome c oxidase (CCO). (d) hCTR, a human homologue of CTR, mediates copper-ion uptake into human cells. CCS delivers copper to cytoplasmic Cu/Zn superoxide dismutase (SOD1). Abbreviations IMM, inner mitochondrial membrane OMM, outer mitochondrial membrane PM, plasma membrane PGV, post Golgi vessel. Reprinted from Harrison et al., 2000. Copyright (2000), with permission from Elsevier Science.

Not all hereditary traits follow the Mendelian patterns expected for chromosomal genes. Some are inherited directly from the maternal cell because their genes are carried in the cytoplasm rather than the nucleus. There are three known locations for cytoplasmic genes the mitochondria, the chloroplasts, and certain other membrane-associated sites.285 286 An example of the last is found in "killer" strains of yeast. Cells with the killer trait release a toxin that kills sensitive cells but are themselves immune. The genes are carried in double-stranded RNA rather than DNA, but are otherwise somewhat analogous to the colicin factors of enteric bacteria (Box 8-D). Similar particles (kfactors) are found in Paramecium.287... 

The term nucleoside was originally proposed by Levene and Jacobs in 1909 for the carbohydrate derivatives of purines (and, later, of pyrimidines) isolated from the alkaline hydrolyzates of yeast nucleic acid. The phosphate esters of nucleosides are the nucleotides, which, in polymerized forms, constitute the nucleic acids of all cells.2 The sugar moieties of nucleosides derived from the nucleic acids have been shown, thus far, to be either D-ribose or 2-deoxy-D-eri/fAro-pentose ( 2-deoxy-D-ribose ). The ribo-nucleosides are constituents of ribonucleic acids, which occur mainly in the cell cytoplasm whereas 2-deoxyribo -nucleosides are components of deoxypentonucleic acids, which are localized in the cell nucleus.3 The nucleic acids are not limited (in occurrence) to cellular components. They have also been found to be important constituents of plant and animal viruses. 

Fig. 1. Schematic overview of copper trafficking and homeostasis inside the yeast cell. The actions of Mad and Ace 1, copper-dependent metalloregulatory transcription factors, control the production of copper import [copper transporter (Ctr) and reductase (Fre)] and detoxification/sequestration [metallothionein (MT)] machineries, respectively. Three chaperone-mediated delivery pathways are shown. Atxl shuttles Cu(I) to the secretory pathway P-type ATPase Ccc2 (right). CCS delivers Cu(I) to the cytoplasmic enzyme copper-zinc superoxide dismutase (SOD) (left). Coxl7 shuttles Cu(I) to cytochrome c oxidase (CCO) in the mitochondria (bottom). Mitochondrial proteins Scol and Sco2 may also play a role in copper delivery to the CuA and CuB sites of CCO. Copper metabolism and iron metabolism are linked through the actions of Fet3, a copper-containing ferroxidase required to bring iron into the cell (lower right) (see text).

Under conditions where copper is in excess in the surrounding medium, the yeast cell s perspective shifts from one of active acquisition of copper ion to one of protecting the cytoplasm from its toxic effects. To... 

Fig. 3A.1 LTSEM images of yeast cells after 24 h of induced autolysis in a model wine system, a Superficial ultrastructure of a yeast cell. b,c Images of fractured empty yeast cells which have lost most of their cytoplasmic content during the autolysis (Maitfnez-Rodrfguez AJ, Polo MC, Carrascosa AV (2001) Int J Food Microbiol 71 45-51. Copyright (2001). Elsevier)...

While the in vitro studies on assembly have provided relatively little information, in vivo data can give us some suggestions on possible pathway of assembly. For a long time it has been known that in rho yeast cells, where cytochrome b is not produced, cytochrome c, is still accumulated in the inner membrane. This agrees with the plasmid studies of overproduction. On the other hand the cytoplasmically synthesized subunits of cytochrome c oxidase accumulate in much lower quantities in the absence of subunits I, II and III, which are mitochondrial products. It is unlikely that this diminished accumulation is due to substantially reduced gene expression. This may indicate that certain subunits are stabilized by their counterparts. 

Carrier-mediated movement of sugars across the plasmalemma of yeasts involves the combination of the sugar with a protein on one side of the plasmalemma, followed by release of the sugar into the cytoplasm on the other side. Such movement is described either as (t) facilitated diffusion, when the movement requires no metabolic energy, or (ii) active transport, which involves the expenditure of metabolic energy. Sugars entering yeast cells by active transport may be accumulated within the cells to a concentration many hundred times the external level. This subject has been reviewed by... 

Nonsense-mediated decay occurs in the cytoplasm of yeast cells. Remarkably, In mammalian cells, there Is evi-... 

Fig.1. [P S/+] is cytoplasmically inherited. Black circles [P 7+] determinant. Yeast cells can grow either as haploids (one copy of each chromosome) or diploids (two copies of each chromosome). When a [P SP] haploid is mated to a [psi ] haploid, the resulting diploid is [P SP], indicating that [P P] is dominant. On sporulation, each nuclear determinant (gray fill and wavy lines) segregates to half the haploid progeny (2 2), and the cytoplasmic [P SP] determinant segregates to all progeny (4 0).

---