Yeast and Genetic Analysis

Some authors prefer to grow yeast for mutation experiments on solid media because, in haploid strains, cells seem to be better separated after growth on solid media than in liquid media. 

Sporulation of diploid yeast cells is achieved both in well-aerated liquid and on the surface of solid sporulation media. Sporulation becomes visible after 12-14 hr, and well-developed asci obtain after about 3 days. The optimal temperature for yeast growth is between 25 and 35°G for sporulation, the lower temperature is preferable. 

If random spore analysis is preferred, digestion is carried on until the ascus wall is completely dissolved. The lipophilic spores tend to cluster 

Tetrad analysis on only partially digested cultures requires little time and has the advantage of reliability. Digestion has to be reduced to a limit which allows a relatively easy bursting of asci with a micromanipulator needle on an agar slab (3% agar in a 0.4% solution of potassium acetate). 

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