Wheat genetic variants

High resolution two-dimensional electrophoresis allows hundreds of proteins to be separated and characterized in submilligram samples of complex protein mixtures. Applications of this method to the analysis of agriculturally important products, including milk, meat, and wheat are reviewed. In a model study we analyzed 100 individual kernels of the wheat cultivar Newton (Triticum aestivum L.) for electrophoretic variants. One variant protein was found in 47 kernels, while three variant proteins occurred together in two of the kernels. The implications of two-dimensional electrophoresis for cultivar identification and the problem of relating electrophoretic protein variants to genetic variants are discussed. 

Gianibelli, M. C., O. R. Larroque, R Chan, B. Margiotta, E. Deambrogio, F. MacRitchie, and D. Lafiandra. 1998. Effect of allelic variants at the Gli-1/ Glu-3 and Gli-2 loci on mixing properties. In Proceedings of the 9th International Wheat Genetics Symposium, vol. 4,154—156. ed. A. E. Slinkard. Saskatchewan, Canada University of Saskatchewan. 

Seilmeier, W., Belitz, H.-D., Wieser, H. Separation and quantitative determination of high-molecular subunits of glutenin from different wheat varieties and genetic variants of the variety Sicco. Z. Lebensm. Unters. Forsch. 192, 124 (1991)... 

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