Weigert stain

The Gram-Weigert stain is used to stain the cyst walls of P. carinii cysts. It also stains fungi and many bacteria. Impression... 

Ehrlich was born in Strehlen, Germany, and attended school in Breslau where an older cousin, Carl Weigert, was a physician at a local hospital. Weigert was researching cell staining with synthetic dyes, a procedure that makes cells more visible under a microscope. Weigert demonstrated the technique for his teenage cousin who was immediately fascinated by the process. 

Fig. 1. Bovine preparations stained with Weigert s resorcin-fuchsin (Partridge and King, 1961).

Ple6nik (1902) was among the first to apply Weigert s chrome-hematoxylin stain to adrenal glands fixed for a prolonged period in potassium dichromate mixtures. He found that the droplets in the adrenal cortex stained intensely after such mordanting, as did those of the interstitial cells in the testis, but that ordinary fat failed to stain. 

Fig. 135. Alveolar macrophages having engulfed powdered diamond. 3-Day old culture of a 3-day old mouse. The preparation was fixed in Carnoy s fluid, stained with iron hematoxylum after Weigert, and mounted in toto in Rhenohistol. Eye-piece Leitz Peri-plan 4x objective Leitz Pv FI oil 70/1.15. Positive phase contrast (from Schiller 1954)...

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