Weak-affinity chromatography

Wikstroem, M. and Ohlson, S., Computer simulation of weak affinity chromatography, J. Chromatogr.,... 

Mouse brain lectin weak affinity chromatography 0-GlcNAc LC-MS/MS MALDI-TOF modified proteins (Vosseller et al. 2006)... 

Vosseller, K., Trinidad, J.C., Chalkley, R.J., Specht, C.G., Thalhammer, A., Lynn, A.J., Snedecor, J.O., Guan, S., Medzihradszky, K.F., Maltby, D.A., Schoepfer, R. and Burlingame, A.L. (2006) 0-Linked N-Acetylglucosamine proteomics of postsynaptic density preparations using lectin weak affinity chromatography and mass spectrometry. Mol. Cell Proteomics 5, 923-934. 

Fig. 1 Analysis of the oligosaccharide (Glc)4 in (a) human urine and (b) serum by weak affinity chromatography. The affinity column was 5 mm LD. x 100 mm and contained immobilized monoclonal antibodies against (Glc)4. Elution was carried out under isocratic conditions at a flow rate of 0.2 mL/min using a pH 7.5, 0.02 M phosphate buffer that contained 0.1 M sodium sulfate. (From Ref. [1].)...

Standh, M. Andersson, H.S. Ohlson, S. Weak affinity chromatography. In Affinity Chromatography Bailon, P. Ehrlich, G.K. Fung, W.J. Berthold, W., Eds. Humana Press Totowa, NJ, 2000 Chapter 2. 

The general principles of weak affinity chromatography (WAC) are discussed in this entry. WAC is a subset of affinity chromatography that makes use of weak interactions for the separation or analysis of chemicals. These conditions create a situation in which isocratic elution can be used to pass an analyte through the colurtm. The theory of WAC is discussed and examples are given of ligands that can be used in this technique. Examples of applications that have made use of WAC are also presented. 

Source From Weak affinity chromatography, in Handbook of Affinity Chromatography... 

Fig. 3 Separation and determination of the enantiomers of ketamine in human plasma by using weak affinity chromatography and a column containing Oj-acid glycoprotein as the stationary phase with detection by mass spectrometry.

Ohlson, S. Zopf, D. Weak affinity chromatography. In Handbook of Affinity Chromatography, Kline, T., Ed. Marcel Dekker New York, 1993 Chapter 11. 

Ohlson, S. Bergstrom, M. Leickt, L. Zopf, D. Weak affinity chromatography of small saccharides with immobilized wheat germ agglutinin and its application to mon- 16. itoring of carbohydrate transferase activity. Bioseparation 1998, 7, 101-105. 

Wikstroem, M. Ohlson, S. Computer simulation of weak affinity chromatography. J. Chromatogr. 1992, 597, 83-92. Bergstrom, M. Ohlson, S. Use of perfusion supports in weak affinity chromatography. Inti. J. BioChromatogr. 2001, 6, 163-172. 

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