Wavelength switch

FIGURE 3 HPLC chromatogram of Drug A and Drug B at single channel (dual wavelength with wavelength switch Xj - X,). 

Little, B.E., Haus, H.A., Foresi, J.S., Kimerling, L.C., Ippen, E.P., and Ripin, D.J., 1998, Wavelength switching and routing using absorption and resonance, IEEE Photon. Technol. Lett. 10(6) 816-818. 

Detector wavelength range, wavelength switching, sensitivity, and resolution (slid width)... 

Pandey, G., Sridhar, M., and Bhalerao, U.T. (1990) Regiospecfic dihydroindoles directly from p-arylethylamines by photoinduced SET reaction one-pot wavelength switch approach to benzo-pyrrolizidines related to mitomycin. Tetrahedron Letters, 31, 5373-5376. 

Che S, Li Q, Huo YS, Chen XH, Bi KS (2010) RP-HPLC simultaneous determination of five triterpenoid acids in different parts of Poria cocos by UV wavelengths switch. Yao Xue Xue Bao 45 494 97... 

Figure 5 Chromatogram from the reversed-phase HPLC separation of retinol (R, 0.734 pg/mL), frans-P-apo-lO -carotenal oxime (ox, internal standard), lutein (L, 0.110 pg/mL), zeaxanthin (Z, 0.051 pg/mL), P-cryptoxanthin (C, 0.060 pg/mL), tratw-lycopene (Ly, 0.124 pg/mL), a-carotene (a, 0.022 pg/mL), fran -P-carotene (P, 0.567 pg/mL), and retinyl palmitate (RP, spiked, 0.297 pg/mL) in human serum using absorbance detection with wavelength switching. Chromatogram of tocol (T, internal standard), S-tocopherol (5, 0.084 pg/mL), y-tocopherol (y, 1.94 pg/mL), and a-tocopherol (a-t, 5.69 pg/mL) using fluorescence detection. (From Ref. 197.)...

Folic acid can be determined in methods designed for multivitamin analysis either from multivitamin preparations (90-93) or from fortified samples such as infant milk (94). In these methods trichloroacetic acid extraction of liquid and powdered infant milk followed by ion pair chromatography with reversed-phase Cig column was applied. Satisfactory separation was achieved with octanesulfonic acid (5 mM) with triethylamine (0.5%) in methanol-water (15 85 v v) at pH 3.6 (Fig. 6). UV detection and wavelength switching were used for six vitamins giving sensitivity of 1 ng folic acid per injection (282 nm for folic acid). Total run time of this isogratic separation was 55 min. This approach has not been reported for nonfortified samples. 

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