Water Sterilization Technologies

Isopropyl P-3-thiogalactopyranoside (IPTG) (Life Technologies) 100 mg/mL in water. Sterilize by filtration through a 0.22-p disposable filter. Store in aliquots at -20°C. 

The CSTR cascade is often used as a substitute for CPFR behavior, and tubular reactors themselves are rarely found in bioprocessing. Exceptions are waste water treatment in oxidation ponds, river analysis (Metcalf and Eddy, 1972), sterilization technology, and fixed-bed reactors filled with immobilized enzymes for bioconversions (Lilly and Dunnill, 1972). 

Solid foods have received little attention. Huber (H25) and the Massachusetts Institute of Technology group (P10) maintain that there is no destruction of water-soluble vitamins when a variety of foods is sterilized. 

Place a new autoclave bag over the end of the closure jaws. Take a sterile swab and knock about 30-50 caps into the bag. Pour on sterile mixture of 80% product plus 20% water. Tie up the bag and leave in a warm place at 22-26°C. Observe for up to one week. If no fermentation occurs, then caps are an unlikely contamination source. If fermentation does occur, then action is required. If results indicate activity, this is evidence that technology needs attention. The most probable identity of the micro-organisms is restricted to a very few in Group 1 (see Table 11.5). 

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