Water model recorded attributes

Moreover, adding small amounts of water to the solvent had no apparent effect on the spin label s mobility, as evidenced by the spectra in Figure 2. EPR spectra recorded in butyl acetate with and without 1% water revealed that the active site conformation was unchanged by water addition even though the enzyme s activity was much higher when 1% water was present. Thus, the increase in activity upon water addition cannot be attributed to a loosening up of the active-site conformation. This result differs from a proposed model of the sequential hydration of dry lysozyme (19), in which restoration of enzyme activity followed a hydration-induced flexibility increase. 

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