Warfarin, HPLC analysis

The Ki for HSA binding to racemic warfarin has been reported for 3-6 pM by various techniques, including frontal analysis and equilibrium dialysis, and is temperature- and pH-dependent. See Loun, B., Hage, D.S. Chiral separation mechanisms in protein-based HPLC columns. 1. Thermodynamic studies of (R)- and (S)-warfarin binding to immobilized human serum albumin. Anal. Chem. 1994, 66, 3814-3822. 

Contemporary confirmatory methods use MS. Most recently, liqrud chromatography-electrospray ionization-mass spectroscopy (LC-EIS-MS) has been reported for the analysis of 10 anticoagulant rodenticides with a quantity limit of about 5 pg/L (Grobosch et al., 2006). Other recent methods use LC-MS-MS for unknown drugs, including warfarin (Marquet et al., 2003), and LC-ESTMS and HPLC UV to detect anticoagulant rodenticides as low as 20 ng on a column (Mesmer and Flurer, 2000). One of the earlier MS methods used a direct-probe technique to detect indandione residues in food animals (Braselton et al., 1992). 

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