Walking and Jumping Were Both Used to Map the Cystic Fibrosis Gene

Walking and Jumping Were Both Used to Map the Cystic Fibrosis Gene 

In many genetic disorders, cystic fibrosis included, the analysis begins before the responsible gene and its protein 

Map of restriction fragment length polymorphisms (RFLPs) closely linked to the cystic fibrosis (CF) gene. The inverted triangle near the right-hand end indicates the location of the AF508 mutation characteristic of most persons with cystic fibrosis disease. (Source  

Adapted from B-S. Kerem, I. M. Rommens, I. A. Buchanan, D. Markiewicz, T. K. Cox, A. Chakravarti, M. Buchwald, and L-C. Tsui, Identification of the cystic fibrosis gene Genetic analysis, Science 245 1075, 1989.) 

This complex analysis would be in vain if one did not have some criterion for knowing when the goal of finding the cystic fibrosis gene was reached. This is where clues from the physiological nature of the condition became useful. In a brilliant strategy a cDNA library was prepared from 

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