Vitro Screening Methods

Although the platinum-amino-acid complexes do not show much promise as cytotoxic agents, these results demonstrated the utility of in vitro screening methods to survey the DNA-binding properties of platinum compounds in a combinatorial manner. Assuming that HMG-domain proteins are involved in the cisplatin mechanism of action, then screening based on the Pt-... 

Clark, C.R., Smith, J.R., Shih, M.L. (1999). Development of an in vitro screening method for evaluating decontamination of sulfur mustard by reactive dermal formulations. J. Appl. Toxicol. 19 S77-81. 

The in vitro screening methods have several advantages over in vivo testing such as the undisputable ethical benefits, a lower cost, and a shorter time to perform the study. Moreover, in vitro methods offer the possibility to identify and investigate specific toxicity mechanisms (such as oxidative stress, pathways involved in NM intracellular uptake, genotoxicity) in a relatively simple system, without the complexity and interferences of several factors present in vivo in a whole organism. 

Shibata Y, Takahashi H, Ishii Y.A convenient in vitro screening method for predicting in vivo drug metabolic clearance using isolated hepatoc 4 es suspended in serum. Drug Metab Dispos 2000 28 1518-1523. 

Witchel, H.J., Milnes, J.T., Mitcheson, J.S., and Hancox, J.C., Troubleshooting problems with in vitro screening of drugs for QT interval prolongation using HERG K+ channels expressed in mammalian cell lines and Xenopus oocytes, J. Pharmacol. Toxicol. Methods, 48, 65-80, 2002. 

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