Vimentin cell culture

These data suggested that carnosine can be drawn into the protein synthesis providing specific regulatory role directed to support of essential genes in active state and to increase cell viability. In agreement with such suggestion, it was found that addition of carnosine to cell cultures promotes their viability and stimulates synthesis of a number of proteins, particularly, vimentin, which takes part in interaction of cytoskeleton with membrane lipid bilayer [63]. 

Fig. 4 Utilization of Morphology Explorer to determine optimal time point of differentiated myotubes. Images of human skeletal muscle cells stained by immunofluorescence of a-myosin heavy chain (a-MHC), Titin, Vimentin, and nuclei by Hoechst 33342 were acquired at 2 days before cell confluence (-2), at cell confluence (0), and at day 3,7,10, and 14 post cell confluence (a-r) Quantitative readouts of cell culture features, obtained from four selected output parameters from the Morphology Explorer bio-application, showed that peak of differentiated myotubes were obtained on day 3 post confluence (s-v)...

Carnosine can affect gene expression. Ikeda et al. (1999) showed that carnosine markedly upregulates vimentin synthesis in cultured rat fibroblasts, while an association between carnosine and vimentin, a cytoskele-tal, intermediate filament protein has been noted in glial cells and neurons (Bonfanti et al., 1999). Interestingly, it has also been shown that the protease, oxidized protein hydrolase (OPH), is coexpressed with vimentin in COS cells (Shimizu et al., 2004). Thus, it is at least possible that carnosine could induce synthesis of OPH in the cultured human fibroblasts and thereby increase the cellular ability to eliminate oxidized... 

Ikeda, D., Wada, C., Yoneda, C., Abe, H., and Watabe, S. (1999). Carnosine stimulates vimentin expression in cultured rat fibroblasts. Cell Struct. Fund. 24, 79-87. 

Hartig, R., Shoeman, R.L., Janetzko, A., Tolstonog, G. and Traub, P. (1998b) DNA-mediated transport of the intermediate filament protein vimentin into the nucleus of cultured cells. J. Cell Sci., Ill, 3573-3584. 

WaUin A, Zhang G, Jones TW, Jaken S, Stevens JL. 1992. Mechanism of the nephrogenic repair response. Studies on prohferation and vimentin expression after 35S-l,2-dichlorovi-nyl-L-cysteine nephrotoxicity in vivo and in cultured proximal tubule epithehal cells. Lab Invest 66(4) 474—484. 

In tissue sections of the porcine pulmonary trunk, endothelial cells were specifically immunola-belled with both the monoclonal and polyclonal antibodies raised against vimentin (Schnittler et al. 1998). A moderate degree of staining intensity was observed in all endothelial cells of the pulmonary trunk. The pulmonary trunk contained a 2- to 2.5-fold higher amount of vimentin than the endocardial endothelium of the right ventricle. Cultured endothelial cells of the porcine pulmonary trunk displayed considerably higher amounts of vimentin than did freshly isolated cells. 

Vimentin, desmin, and smooth muscle cell a ac-tin were expressed in all cultures of GRX murine hepatic stellate cells (Guma et al. 2001). Glial fibrillary acidic protein showed a heterogeneous intensity of expression and did not form a filamentous cytoskeletal network, showing a distinct punctuate cytoplasmic distribution. When activated by inflammatory mediators, GRX cells increased expression of desmin and glial fibrillary acidic protein. Retinol-mediated induction of the lipocyte phenotype elicited a strong decrease of intermediate filament protein expression and the collapse of the filamentous structure of the cytoskeleton. 

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