Vessels and Equipment for Solid-Phase Synthesis

The unique feature of solid-phase synthesis is the elimination of unconsumed reactants and secondary products by filtration. The latter is possible because chain 

FIGURE 5.3 Reaction vessels for solid-phase synthesis. (A) 10-300-mL vessel (0.5-10 g of resin) affixed to a rotating-arc shaker (B) 0.5-8-L (20-200 mm in diameter) cylindrical container with stirrer for up to 500 g of resin (C) Ananth vessel with two 80-mL chambers.10 

FIGURE 5.4 Schematic representation of a continous-flow system for the solid-phase synthesis of peptides. Solvent is forced through the system by a pump. The support is in the form of a column that is stationary. A reaction is monitored by measuring the change in absorbance of the solvent stream. 

RB Merrifield. Solid phase synthesis. I. The synthesis of a tetrapeptide. J Am Chem Soc 85, 2149, 1963. 

RB Merrifield, JM Stewart, N Jemberg. Instrument for automated synthesis of peptides. Anal Chem 38, 1905, 1966. 

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