Ventricular wedge preparations

Additionally, the isolated heart can be used to measure chronotropic or inotropic effects. There are several additional ex vivo assays like the sinoatrial node preparation [76], left ventricular wedge preparation [77] available which address specific questions. All of the ex vivo studies mentioned above with the... 

Yan G-X, Shimizu W, Antzelevitch C (1998) Characteristics and distiibutimi of M cells in arterially perfused canine left ventricular wedge preparation. Circulation 98 1921-1927 Zahler R, Sun W, Ardito T, Zhang ZT, Kocsis ID, Kashgarian M (1996) The a3 isoform protein of the Na+, K+-ATPase is associated with the sites of cardiac and neuromuscular impulse transmission. Circ Res 78 870-879... 

Wang D, Patel C, Cui C, YanGX (Aug 2008). Preclinical assessment of drug-induced proarrhythmias role of the arterially perfused rabbit left ventricular wedge preparation. Pharmacol Ther 119(2) 141-151. 

Left ventricular wedge preparation Langendorff preparation -In Vivo... 

While the isolated coronary artery-perfused ventricular wedge preparations have been shown to have a high specificity and selectivity for human torsadogens (Antzelevitch 2004 Liu et al. 2006), perceived hmitations include the in vitro setting, the requirement for technical expertise and familiarity and the consequence that the model is not fit for high-throughput SP. 

The M cell is a unique myocardial cell type found in the deeper layers of the ventricular wall (Antzelevitch et al. 1999). These cells respond more sensitively to agents that block hERG channels and, as such, contribute to possible drug-induced transmural heterogeneity of ventricular repolarization and thereby the proarrhythmic potential. The perfused myocardial wedge preparation is design to allow the study of transmural differences in drug action on the action potential and may therefore provide a better assessment of possible proarrhythmic potential of a test article. 

Arterially perfused wedge left ventricular preparations... 

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