Vector ampicillin resistance

Clones with ampicillin resistance but without kanamycin resistance are examined with the CloneChecker System. By this antibiotic selection, the undesired vector heterodimer is removed. If both of the clones are false, additional clones are analyzed using the same protocol. When appropriate clones are obtained for almost all of the genes, they are inoculated from the ampicillin plates to prepare plasmids in a 96-well format by using the Wizard SV 96 Plasmid DNA Purification System see Note 6). 

YEP24 Plasmid E. coli, yeast Ampicillin resistance. Yeast shuttle vector... 

FIGURE 17.5 Bacterial plasmid vector FliTrx used for random peptide display library [10]. FliC, flagellin gene trxA, thyoredoxin PPL, PL, promoter Col El, E. coli origin of replication ampicillin, gene for ampicillin resistance. 

Figure 15.4 pBR322 plasmid vector showing antibiotic resistance genes (tetR, tetracycline resistance ampR, ampicillin resistance) and the direction of their transcription. Some restriction sites are also shown, as well as the effect of cloning a new sequence into the Hindlll site. 

CA over-expression vector was introduced into Synechococcus sp. PCC7942 resulting ampicillin resistant transformants. SouAem blot analysis revealed the existence of introduced gene (icfA) in transformants. The antiserum against icfA was raised and was used for Western blot analysis. Western blot analysis revealed increased levels of the 30kDa icfA protein in eleven transformants, but not in five vector-control clones (Fig.3). 

The pUC vectors contain an ampicillin-resistance gene and a multiple cloning site at the 5 -end of the lacZ gene. Insertional inactivation of lacZ allows... 

Fig. 17.11. Production of human insulin in E. coli. Amp = the gene for ampicillin resistance. The presence of Amp allows bacterial cells that contain the vector to grow in the presence of ampicillin. Cells lacking the Amp gene die in the presence of ampicillin. Because E. coli cannot process preproinsulin, a synthetic scheme was developed whereby each individual chain of insulin was expressed, produced, and purified, and then the two chains were linked together in a test tube.

Fig. 6. Fraction of ampicillin-resistant clones of E. coli MG 1655 (circles) and a chemostat-selected descendant (squares) from serial batch cultivations in ampicillin-free minimal medium. Both strains harbor the expression vector pCSS4-p for periplasmic production of the recombinant a-amylase of B. stearothermophilus. Reproduced with permission from Weikert etal. [137]...

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